Molecular Characterisation of Pasteurella Multocida Strains Isolated from Calves by Pulsed-field Gel Electrophoresis

Seventy-two Pasteurella multocida strains collected from calves with bronchopneumonia were examined. An analysis of the polymorphism of DNA restrictive fragments with the use of the pulsed-field gel electrophoresis technique of contour-clamped homogeneous electric field electrophoresis was made. Philogenetic relatedness among the examined strains was calculated by the unweighted pair-group method using arithmetic averages . The Polish strains originating from the cases of enzootic bronchopneumonia in calves demonstrated a marked diversity of the genomic DNA. The restrictive patterns of all taxonomic groups of P. multocida occurring in calves in Poland were characteristic for the groups containing several isolates or for single strains. Only P. canis biotype 2 isolates revealed a close relatedness at a genotype level.